INTRODUCTION: Immunofluorescence is an immunological technique which is used to identify antibodies against antigens that are bound to tissues or those circulating in body fluids.

AIM:

 To determine the utility of IF in diagnosing vesiculobullous lesions of skin.

 To correlate IF with histopathological findings.

OBJECTIVES:

 To study the histopathological and immunofluorescence findings of vesiculobullous lesions of skin.

STUDY DESIGN:

 A cross sectional observational study conducted in department of pathology, ASRAM from Jan 2019 to Jan 2022.

MATERIALS AND METHODS:

 This is a cross sectional observation study. The present study will be conducted in the pathology department, Asram medical college, Eluru from Jan 2019 to Jan 2022. The cases received during this period and diagnosed as vesiculobullous lesion in histopathology will be included in our study.

 Two skin biopsies for each case- one case in formalin for routine histopathological examination and other in phosphate buffer solution for the IF studies- will be received.

 The lesions will be diagnosed by both light microscopy and immunofluorescence study . The cases that are positive with the immunofluorescence technique overall sensitivity of IF technique in % along with specificity, ppv and npv will be calculated in the study.
 When histopathological and IF findings are correlated, statistical significance with two-tailed p= will be obtained.

INCLUSION CRITERIA:

 All the biopsy proven cases of vesiculobullous lesions during this period.

EXCLUSION CRITERIA:

 Patient consent not given

STATISTICAL ANALYSIS: Specificity, sensitivity, ppv and npv .P value will be obtained